• Biology
  • Published 2014

Detection and quantification of dengue virus 2 in culture supernatant and plasma of children by qPCR using a commercial kit and the EcoTM System-Illumina device

@inproceedings{Celis2014DetectionAQ,
  title={Detection and quantification of dengue virus 2 in culture supernatant and plasma of children by qPCR using a commercial kit and the EcoTM System-Illumina device},
  author={Federico Perdomo Celis and Piedad Perilla and Doris Martha Salgado and Carlos Fernando Narv{\'a}ez},
  year={2014}
}
Methods for Dengue virus (DENV) diagnosis in endemic areas are greatly needed. One of them is the real-time polymerase chain reaction (qPCR) that also enables to quantitate the viral genome. Kits of qPCR for DENV are expensive and restrict their use to a small number of qPCR devices, which limits the application of the technique. Here, we evaluated the performance of a commercial kit of qPCR to DENV-2 detection on a locally available qPCR device (Eco TM System, Illumina), not cited by the kit… CONTINUE READING

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