Detection and quantification of chimerism by droplet digital PCR.

@article{George2013DetectionAQ,
  title={Detection and quantification of chimerism by droplet digital PCR.},
  author={David George and Juliann Czech and Bobby Anak John and Min Yu and Lawrence J. Jennings},
  journal={Chimerism},
  year={2013},
  volume={4 3},
  pages={102-8}
}
Accurate quantification of chimerism and microchimerism is proving to be increasingly valuable for hematopoietic cell transplantation as well as non-transplant conditions. However, methods that are available to quantify low-level chimerism lack accuracy. Therefore, we developed and validated a method for quantifying chimerism based on digital PCR technology. We demonstrate accurate quantification that far exceeds what is possible with analog qPCR down to 0.01% with the potential to go even… CONTINUE READING

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