Detection and quantification of botulinum neurotoxin type a by a novel rapid in vitro fluorimetric assay.

@article{Poras2009DetectionAQ,
  title={Detection and quantification of botulinum neurotoxin type a by a novel rapid in vitro fluorimetric assay.},
  author={Herv{\'e} Poras and Tanja Ouimet and Sou-Vinh Orng and Marie-Claude Fourni{\'e}-Zaluski and Michel Robert Popoff and B. Roques},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 13},
  pages={4382-90}
}
Botulinum neurotoxin type A (BoNT/A), the most poisonous substance known to humans, is a potential bioterrorism agent. The light-chain protein induces a flaccid paralysis through cleavage of the 25-kDa synaptosome-associated protein (SNAP-25), involved in acetylcholine release at the neuromuscular junction. BoNT/A is widely used as a therapeutic agent and to reduce wrinkles. The toxin is used at very low doses, which have to be accurately quantified. With this aim, internally quenched… CONTINUE READING
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