Detection and quantification by real-time RT-PCR of hepatitis A virus from inoculated tap waters, salad vegetables, and soft fruits: characterization of the method performances.

@article{Dubois2007DetectionAQ,
  title={Detection and quantification by real-time RT-PCR of hepatitis A virus from inoculated tap waters, salad vegetables, and soft fruits: characterization of the method performances.},
  author={Eric P. Dubois and Catherine Hennechart and G. Merle and Christian Burger and Nadia Hmila and St{\'e}phanie Ruelle and Sylvie Perelle and Virginie Ferr{\'e}},
  journal={International journal of food microbiology},
  year={2007},
  volume={117 2},
  pages={141-9}
}
Water, salad vegetables and fruits exposed to fecal contamination may cause outbreaks of hepatitis A. A protocol of viral concentration by filtration on electronegative membrane filter and a protocol based on a viral elution in Tris-glycine buffer, pH 9.5 with concentration by polyethylene glycol precipitation were associated with real-time, reverse transcriptase-PCR to detect hepatitis A virus (HAV) artificially inoculated in 2 l of tap water, or on 25 g of fruits or salad vegetables. These… CONTINUE READING

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