Detection and monitoring of anaerobic rumen fungi using an ARISA method.

  title={Detection and monitoring of anaerobic rumen fungi using an ARISA method.},
  author={Stuart Edward Denman and Matthew J. Nicholson and Jayne L Brookman and Michael K. Theodorou and Christopher S. McSweeney},
  journal={Letters in applied microbiology},
  volume={47 6},
AIM To develop an automated ribosomal intergenic spacer region analysis (ARISA) method for the detection of anaerobic rumen fungi and also to demonstrate utility of the technique to monitor colonization and persistence of fungi, and diet-induced changes in community structure. METHODS AND RESULTS The method could discriminate between three genera of anaerobic rumen fungal isolates, representing Orpinomyces, Piromyces and Neocallimastix species. Changes in anaerobic fungal composition were… CONTINUE READING

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