Detection and measurement of total bilirubin in serum, with use of surfactants as solubilizing agents.

@article{Pearlman1974DetectionAM,
  title={Detection and measurement of total bilirubin in serum, with use of surfactants as solubilizing agents.},
  author={F. C. Pearlman and Reiko T. Lee},
  journal={Clinical chemistry},
  year={1974},
  volume={20 4},
  pages={
          447-53
        }
}
We describe a new method for measuring total bilirubin in serum. Nonionic, cationic, or anionic surfactants can be used as solubilizing agents to promote the diazo coupling of indirect-reacting bilirubin. A representative surfactant—azobilirubin system is illustrated, in which absorbance is maximum at 560 nm and linear to a concentration of 200 mg of bilirubin per liter. At pH 2.5 and in the presence of Du ponol (an anionic surfactant), bilirubin is completely coupled within 6 mm at 37 °C. All… CONTINUE READING

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