Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation

@inproceedings{ContrerasAguilar2017DetectionAM,
  title={Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation},
  author={Mar{\'i}a Dolores Contreras-Aguilar and Fernando Tecles and Silvia Mart{\'i}nez-Subiela and Dami{\'a}n Escribano and L. J. Bernal and Jos{\'e} J Cer{\'o}n},
  booktitle={BMC veterinary research},
  year={2017}
}
BACKGROUND Salivary alpha-amylase (sAA) is considered a biomarker of sympathetic activation in humans, but there is controversy regarding the existence of sAA in dogs. The hypothesis of this study was that sAA exists in dogs and it could change in situations of sympathetic stimulation. Therefore, the aims of this study were: 1) to demonstrate the presence of alpha-amylase in saliva of dogs by Western-Blot, 2) to validate an spectrophotometric method for the measurement of sAA activity and 3) to… CONTINUE READING
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