Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments

Abstract

Various DNA subfragments were derived from miniF DNA by complete or partial PstI cleavage, and cloned in the plasmid vectors pBR322 or λdv1. The recombinant plasmids obtained were introduced into an Escherichia coli minicell-producing strain, and the plasmid-coded proteins were radiolabeled and analyzed by gel electrophoresis. Six miniF-encoded proteins… (More)
DOI: 10.1007/BF00331850

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@article{Komai1982DetectionAM, title={Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments}, author={Nobuhisa Komai and Tsutomu Nishizawa and Yasuhiko Hayakawa and Tomoaki Murotsu and Kenichi Matsubara}, journal={Molecular and General Genetics MGG}, year={1982}, volume={186}, pages={193-203} }