Detection and localization of syntrophic propionate-oxidizing bacteria in granular sludge by in situ hybridization using 16S rRNA-based oligonucleotide probes.

@article{Harmsen1996DetectionAL,
  title={Detection and localization of syntrophic propionate-oxidizing bacteria in granular sludge by in situ hybridization using 16S rRNA-based oligonucleotide probes.},
  author={Hermie J. M. Harmsen and Harry M. P. Kengen and Antoon D. L. Akkermans and Alfons J M Stams and Willem M de Vos},
  journal={Applied and environmental microbiology},
  year={1996},
  volume={62 5},
  pages={1656-63}
}
In situ hybridization with fluorescent oligonucleotides was used to detect and localize microorganisms in the granules of two lab-scale upflow anaerobic sludge blanket reactors that had been fed for several months with either sucrose or a mixture of volatile fatty acids. Sections of the granules were hybridized with 16S rRNA-targeted oligonucleotide probes for Bacteria, Archaea, specific phylogenetic groups of methanogens, and two syntrophic propionate-oxidizing strains, MPOB and KOPROP1. Cells… CONTINUE READING
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