Detection and identification of mycobacteria by mycolic acid analysis of sputum specimens and young cultures.

@article{ViaderSalvad2007DetectionAI,
  title={Detection and identification of mycobacteria by mycolic acid analysis of sputum specimens and young cultures.},
  author={Jos{\'e} Mar{\'i}a Viader-Salvad{\'o} and Carmen Amelia Molina-Torres and Martha Guerrero-Olazar{\'a}n},
  journal={Journal of microbiological methods},
  year={2007},
  volume={70 3},
  pages={
          479-83
        }
}
Ziehl-Neelsen acid-fast staining and mycolic acid analysis of concentrated samples and Middlebrook 7H9 cultures were carried out on 127 sputum specimens to evaluate a rapid method for detecting and identifying mycobacteria by analyzing fluorescent derivatives of mycolic acids in concentrated sputum specimens and in Middlebrook 7H9 cultures and compare with mycobacterial detection using Lowenstein-Jensen (LJ) cultures. All samples were classified into five groups according to the number of acid… CONTINUE READING