Detection and identification of human papillomavirus using a PCR-restriction fragment mass polymorphism assay.

@article{Lee2011DetectionAI,
  title={Detection and identification of human papillomavirus using a PCR-restriction fragment mass polymorphism assay.},
  author={Jin Kyung Lee and Y J Hong and Tae Hyun Um and Eun Hee Lee and Hyun-sook Chi and Jae Soo Koh and Hyeon Woo Yim and Young Joo Cha},
  journal={Molecular medicine reports},
  year={2011},
  volume={4 4},
  pages={645-50}
}
A polymerase chain reaction-restriction fragment mass polymorphism (PCR-RFMP) assay protocol using PGMY09/11 primers for the detection and identification of human papillomavirus (HPV) has recently been developed. The present study evaluated the analytical sensitivity and clinical utility of HPV genotyping employing PCR-RFMP as compared to direct sequencing. Serial dilutions of cloned HPV DNA were analyzed in order to assess the limit of detection (LOD) and three sets of HPV clone mixtures… CONTINUE READING

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