Detection and identification of human influenza viruses by the polymerase chain reaction.

@article{Zhang1991DetectionAI,
  title={Detection and identification of human influenza viruses by the polymerase chain reaction.},
  author={Wei Zhang and David H. Evans},
  journal={Journal of virological methods},
  year={1991},
  volume={33 1-2},
  pages={165-89}
}
A series of oligonucleotide primers are described which hybridize to conserved regions of influenza virus cDNA and prime DNA synthesis in Taq polymerase catalyzed amplification reactions (PCR). Primers were designed to hybridize as nested pairs and, following a two-step amplification, produce uniquely sized DNA fragments diagnostic for viral type and subtype. Influenza A and B matrix-protein genes and the influenza C haemagglutinin gene were targets for the type-specific primers. Subtype… CONTINUE READING

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