Detection and identification of heme c-modified peptides by histidine affinity chromatography, high-performance liquid chromatography-mass spectrometry, and database searching.

Abstract

Multiheme c-type cytochromes (proteins with covalently attached heme c moieties) play important roles in extracellular metal respiration in dissimilatory metal-reducing bacteria. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) characterization of c-type cytochromes is hindered by the presence of multiple heme groups, since the heme c modified… (More)
DOI: 10.1021/pr3007914

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Cite this paper

@article{Merkley2012DetectionAI, title={Detection and identification of heme c-modified peptides by histidine affinity chromatography, high-performance liquid chromatography-mass spectrometry, and database searching.}, author={Eric D. Merkley and Brian J. Anderson and Jea Hun Park and Sara Mae Belchik and Liang Shi and Matthew E. Monroe and Richard D Smith and Mary S. Lipton}, journal={Journal of proteome research}, year={2012}, volume={11 12}, pages={6147-58} }