Detection and identification of bacterial DNA in semen.

  title={Detection and identification of bacterial DNA in semen.},
  author={Ann A. Kiessling and Bryan M Desmarais and Hui-Zhong Yin and Joseph R. Loverde and Robert C Eyre},
  journal={Fertility and sterility},
  volume={90 5},
OBJECTIVE To detect and identify bacteria in semen by sequencing polymerase chain reaction (PCR)-amplified ribosomal RNA gene regions (rDNAs). DESIGN Bacterial rDNAs were detected by PCR amplification of semen DNA. Conditions were adjusted to detect only abundant organisms, no fewer than 20,000 bacteria/mL of semen. SETTING Clinical andrology laboratory… CONTINUE READING