Detection and identification of avian mycoplasmas by polymerase chain reaction and restriction fragment length polymorphism assay.

@article{Kiss1997DetectionAI,
  title={Detection and identification of avian mycoplasmas by polymerase chain reaction and restriction fragment length polymorphism assay.},
  author={Ildik{\'o} Kiss and Katalin Matiz and Eva J Kaszanyitzky and Y Ch{\'a}vez and Karl Erik Johansson},
  journal={Veterinary microbiology},
  year={1997},
  volume={58 1},
  pages={23-30}
}
The polymerase chain reaction (PCR) with primers complementary to the 16S rRNA genes was used to detect avian mycoplasmas. A primer pair designed for the detection of human and rodent mycoplasmal species was examined for its ability to detect the most important avian mycoplasmas. After testing the respective reference strains, we found that Mycoplasma iowae, Mycoplasma meleagridis and Mycoplasma synoviae could be detected by PCR with this primer pair, and distinction could be made among them by… CONTINUE READING