Detection and identification of Rickettsia helvetica and Rickettsia sp. IRS3/IRS4 in Ixodes ricinus ticks found on humans in Spain

  title={Detection and identification of Rickettsia helvetica and Rickettsia sp. IRS3/IRS4 in Ixodes ricinus ticks found on humans in Spain},
  author={Pedro Fern{\'a}ndez-Soto and Ricardo P{\'e}rez-S{\'a}nchez and Antonio Encinas-Grandes and R Sanz},
  journal={European Journal of Clinical Microbiology and Infectious Diseases},
New Rickettsia spp. are continuously being isolated from ticks around the world, but in most cases their pathogenicity remains to be determined. Some rickettsiae first thought to be nonpathogenic have later been associated with human disease, such as Rickettsia slovaca [1], Rickettsia helvetica [2–4], Rickettsia aeschlimannii [5] and, more recently, the Spanish strain Bar29 (Rickettsia massiliae genogroup), which seems to play a role in the pathogenesis of Mediterranean spotted fever [6]. There… 

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Ixodes ricinus ticks are reservoir hosts for Rickettsia helvetica and potentially carry flea-borne Rickettsia species

It is proposed that I. ricinus is a major reservoir host for R. helvetica, and that vertebrate hosts play important roles in the further geographical dispersion of rickettsiae.

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Host-seeking Ixodes ricinus (L.) ticks were collected systematically at selected sites in southern Germany, including a large city park in Munich, to show that R. helvetica is widespread throughout the study region and could result in a threat to public health in areas of high prevalence.

Spotted fever group Rickettsia in ticks from southeastern Spain natural parks

  • F. Márquez
  • Biology
    Experimental and Applied Acarology
  • 2008
During an 8-years study, 1246 ticks belonging to 13 species in Jaen province (Andalusia) were collected and analysis of amplicons of gltA, ompA and ompB genes revealed that Ixodes ricinus were infected with R. monacensis, including strain IRS3, and R. helvetica was found.

Prevalence of tick-borne zoonotic bacteria in questing adult ticks from northern Spain.

A total of 691 questing adult ixodid ticks of the genera Ixodes, Haemaphysalis, Dermacentor, and Rhipicephalus were tested by polymerase chain reaction (PCR) and reverse line blot (RLB) for the

Detection and prevalence of Anaplasma phagocytophilum and Rickettsia helvetica in Ixodes ricinus ticks in seven study areas in Sweden

The results show that both pathogens occurred in all seven locations, indicating that they are prevalent in Sweden and should be considered etiological agents in patients recently bitten by ticks.



Genetic Identification of Rickettsiae Isolated from Ticks in Japan

A new genotype of unknown pathogenicity, genotype AT, that was isolated from Amblyomma testudinarium ticks and that is closely related to a Slovakian genotype obtained from Ixodes ricinus ticks is identified.

Which Spotted Fever Group Rickettsia Are Present in Dermacentor marginatus Ticks in Spain?

T ticks collected during 1999 − 2001 in Jaén and La Rioja (Spain) were identified using molecular techniques and restricted patterns indicate that two rickettsiae were present in the studied samples: R. slovaca and Rickettsia sp.

Identification of Borrelia burgdorferi sensu lato, Anaplasma and Ehrlichia Species, and Spotted Fever Group Rickettsiae in Ticks from Southeastern Europe

The results of this study show that many tick-borne diseases are most probably endemic in the Balkan area and suggest that there is a considerable chance for simultaneous transmission oftick-borne pathogens to human beings.

Genetic Diversity of Bacterial Agents Detected in Ticks Removed from Asymptomatic Patients in Northeastern Italy

A total of 360 ticks were removed from 353 asymptomatic subjects in Belluno Province, Italy and surrounding areas, from 1998 to 2001 and all genera members of the Anaplasmataceae, including Rickettsia, were used.

Characterization of a New Spotted Fever Group Rickettsia Detected in Ixodes ricinus (Acari: Ixodidae) Collected in Slovakia

Phylogenetic relationships inferred from the comparison of these sequences with those of other members of the genus Rickettsia indicate that IRS3 and IRS4 constitute a new rickettsial genotype and form a separate cluster among the spotted fever group ricksettsiae.

Phylogenetic Analysis of Spotted Fever Group Rickettsiae Based on gltA, 17‐kDa, and rOmpA Genes Amplified by Nested PCR from Ticks in Japan

The gltA genes amplified from the ticks were classified into 9 genotypes (I to IX) by the difference in nucleotide sequences, and genotypes I, III, and IV (detected from H. formosensis, H. hystricia, and I. ovatus) are genetically close with each other, but rickettsiae of the same property still have not been isolated from ticks anywhere in the world.

Serosurvey among Mediterranean spotted fever patients of a new spotted fever group rickettsial strain (Bar29)

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New Rickettsiae in ticks collected in territories of the former soviet union.

Dermacentor nuttallii from Siberia, Rhipicephalus sanguineus from Crimea, and Rh. pumilio from the Astrakhan region were infected with Rickettsia sibirica (12%), R. conorii (8%), and the Astrakhan

Aneruptive Fever Associated with Antibodies to Rickettsia helvetica in Europe and Thailand

Eight patients from France, Italy, and Thailand had serological evidence of Rickettsia helvetica infection, which presented as a mild disease in the warm season and was associated with fever, headache, and myalgia but not with a cutaneous rash.