Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction.

  title={Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction.},
  author={Cappucine Picard and C{\'e}cile Ponsonnet and Eric Paget and Xavier Nesme and Pascal Simonet},
  journal={Applied and environmental microbiology},
  volume={58 9},
In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a step particularly critical for some microorganisms which resist classical techniques, we used small soil samples (100 mg) and various lytic treatments, including sonication, microwave heating, and… CONTINUE READING
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