Detection and differentiation of filarial parasites by universal primers and polymerase chain reaction-restriction fragment length polymorphism analysis.

@article{Nuchprayoon2005DetectionAD,
  title={Detection and differentiation of filarial parasites by universal primers and polymerase chain reaction-restriction fragment length polymorphism analysis.},
  author={Surang Nuchprayoon and Alisa Junpee and Yong Poovorawan and A L Scott},
  journal={The American journal of tropical medicine and hygiene},
  year={2005},
  volume={73 5},
  pages={895-900}
}
Filarial nematode parasites are a serious cause of morbidity in humans and animals. Identification of filarial infection using traditional morphologic criteria can be difficult and lead to misdiagnosis. We report on a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)-based method to detect and differentiate a broad range of filarial species in a single PCR. The first internal transcribed spacer 1 (ITS1) along with the flanking 18S and 5.8S ribosomal DNA (rDNA) were… CONTINUE READING
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