Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR.

@article{Bubert1999DetectionAD,
  title={Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR.},
  author={Andreas Bubert and Ingeborg Hein and Marcus Rauch and Angelika Lehner and B Yoon and W. Goebel and Michael Wagner},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 10},
  pages={4688-92}
}
The iap gene encodes the protein p60, which is common to all Listeria species. A previous comparison of the DNA sequences indicated conserved and species-specific gene portions. Based on these comparisons, a combination consisting of only five different primers that allows the specific detection and differentiation of Listeria species with a single… CONTINUE READING