Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence.

@article{Zhao2005DetectionAC,
  title={Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence.},
  author={Hongtao Zhao and Joy Joseph and Henry M. Fales and Edward A. Sokoloski and Rodney L Levine and Jeannette V{\'a}squez-Vivar and Balaraman Kalyanaraman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 16},
  pages={5727-32}
}
Here we report the structural characterization of the product formed from the reaction between hydroethidine (HE) and superoxide (O(2)(.-)). By using mass spectral and NMR techniques, the chemical structure of this product was determined as 2-hydroxyethidium (2-OH-E(+)). By using an authentic standard, we developed an HPLC approach to detect and quantitate the reaction product of HE and O(2)(.-) formed in bovine aortic endothelial cells after treatment with menadione or antimycin A to induce… CONTINUE READING
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