Detection and characterization of human Ureaplasma species and serovars by real-time PCR.

@article{Xiao2010DetectionAC,
  title={Detection and characterization of human Ureaplasma species and serovars by real-time PCR.},
  author={Li Xiao and John I. Glass and Vanya Paralanov and Shibu Yooseph and Gail H. Cassell and Lynn B. Duffy and Ken B. Waites},
  journal={Journal of clinical microbiology},
  year={2010},
  volume={48 8},
  pages={2715-23}
}
We designed primers and probes for the detection and discrimination of Ureaplasma parvum and U. urealyticum and their 14 serovars by real-time PCR. The analytical sensitivity and specificity of the multiplex species-specific PCR were determined by testing corresponding American Type Culture Collection (ATCC) type strains, 47 other microbial species, and human genomic DNA. The limits of the multiplex PCR were 2.8 x 10(-2) CFU/microl PCR mixture for detecting U. parvum and 4.1 x 10(-2) CFU/microl… CONTINUE READING

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