Detection and characterization of defective interfering RNAs associated with the cocksfoot mottle sobemovirus

@article{Makinen2007DetectionAC,
  title={Detection and characterization of defective interfering RNAs associated with the cocksfoot mottle sobemovirus},
  author={K. Makinen and E. Generozov and N. Arshava and A. Kaloshin and S. Morozov and S. Zavriev},
  journal={Molecular Biology},
  year={2007},
  volume={34},
  pages={291-296}
}
A new RNA of about 900 nt was found in the virions of cocksfoot mottle virus (CfMV) and in infected plants by RNA hybridization and RT-PCR. Structural features suggested that this RNA is a defective interfering RNA (diRNA). The CfMV diRNA was shown to consist of a 35-nt 5′-terminal genomic region, which formed a hairpin, and a 3′-terminal genomic region, which included the coat protein (CP) gene lacking the first 120 nt.In vitro translation of the diRNA started at the third Met codon to produce… Expand

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