Detection and characterization of 1B/1R translocations in hexaploid wheat

@article{HeslopHarrison1990DetectionAC,
  title={Detection and characterization of 1B/1R translocations in hexaploid wheat},
  author={J S Heslop-Harrison and Andrew Rowland Leitch and Trude Schwarzacher and Kesara Anamthawat-j{\'o}nsson},
  journal={Heredity},
  year={1990},
  volume={65},
  pages={385-392}
}
Total genomic DNA from rye was labelled with biotin and used as a probe for in situ hybridization to show the sizes and translocation points of the rye chromosome segments in five wheat varieties which carry a translocation between wheat chromosome IB and the short arm of rye chromosome IR (1B/1R). All the translocation breakpoints were at, or very near to, the centromere. Using genomic DNA to block some cross-hybridization, little signal from hybridization between the rye probe and wheat… CONTINUE READING
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