Detection and characterisation of bluetongue virus using the polymerase chain reaction.

@article{McColl1991DetectionAC,
  title={Detection and characterisation of bluetongue virus using the polymerase chain reaction.},
  author={Kenneth A. McColl and Allan R. Gould},
  journal={Virus research},
  year={1991},
  volume={21 1},
  pages={
          19-34
        }
}
Pairs of oligodeoxynucleotide primers whose sequences were based on those of RNA segment 3 that encodes the bluetongue virus serogroup-reactive protein VP3, were synthesized for three BTVs from different geographic regions of the world and for seven Australian orbiviruses. Each pair of primers was then tested for the synthesis of cDNA and in subsequent polymerase chain reactions (PCR) with all ten virus groups. All primers were serogroup-specific at low or high stringency. One pair of primers… CONTINUE READING

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