Detection and Quantitation of Circulating Human Irisin by Tandem Mass Spectrometry.

@article{Jedrychowski2015DetectionAQ,
  title={Detection and Quantitation of Circulating Human Irisin by Tandem Mass Spectrometry.},
  author={Mark P. Jedrychowski and C. Wrann and J. Paulo and Kaitlyn K. Gerber and John Szpyt and M. Robinson and K. Nair and S. Gygi and B. Spiegelman},
  journal={Cell metabolism},
  year={2015},
  volume={22 4},
  pages={
          734-740
        }
}
Exercise provides many health benefits, including improved metabolism, cardiovascular health, and cognition. We have shown previously that FNDC5, a type I transmembrane protein, and its circulating form, irisin, convey some of these benefits in mice. However, recent reports questioned the existence of circulating human irisin both because human FNDC5 has a non-canonical ATA translation start and because of claims that many human irisin antibodies used in commercial ELISA kits lack required… Expand
News: Circulating irisin confirmed by mass spectrometry
  • Tim Geach
  • Medicine
  • Nature Reviews Endocrinology
  • 2015
Irisin was first identified as a hormone secreted by muscle in response to exercise, but the existence of this myokine has been controversial. In a new paper, the investigators who first identifiedExpand
Detection and quantitation of irisin in human cerebrospinal fluid by tandem mass spectrometry
TLDR
Quantitative mass spectrometry unequivocally demonstrated the presence of the glycosylated form of irisin in human cerebrospinal fluid as well as significant individual differences in men over 80 years of age with diseases. Expand
Circulating Levels of Irisin in Hypopituitary and Normal Subjects
TLDR
The correlation between irisin and adiposity related factors suggests that that in the case of this clinical model, irisin is regulated by adiposity and not by GH. Expand
The Controversial Role of Irisin in Clinical Management of Coronary Heart Disease
TLDR
A critical introduction to the latest researches concerning the relationship between irisin and coronary heart disease, which includes atherosclerosis, stable angina pectoris and acute coronary syndromes is made. Expand
Evaluation of Serum Levels of Irisin and Nesfatin-1 in Patients with Migraine without Aura
TLDR
A preliminary assessment to determine serum irisin and nesfatin-1 levels in migraine without aura patients and examine whether these data could help with the identification or evaluation of the disease. Expand
The "irisin system": From biological roles to pharmacological and nutraceutical perspectives.
TLDR
Preclinical and clinical evidence demonstrates the ability of natural and synthetic molecules to interfere with this metabolic pathway of irisin and its serum concentration, which is a challenging issue for biomedical research. Expand
The Effect of Irisin as a Metabolic Regulator and Its Therapeutic Potential for Obesity
TLDR
The existing unresolved issues and controversies about irisin are discussed, including the transcription of the irisin precursor FNDC5 gene in humans, the cleavage site of the yet unknown proteolytic enzyme that cleaves irisin from F NDC5, and the reliability of irisin levels measured with available detection methods. Expand
Physiology and role of irisin in glucose homeostasis
TLDR
This Review presents the current knowledge on the physiology of irisin and its role in glucose homeostasis, and describes the mechanisms involved in the synthesis, secretion, circulation and regulation of irisin, and the controversies regarding the measurement. Expand
Irisin: Still chasing shadows
TLDR
There is a greater transcript diversity of human FNDC5 than currently annotated, but no indication of the expression of transcripts leading to a truncated form of irisin, which represents an explicit warning against measuring circulating irisin using available methods. Expand
Plasma irisin is elevated in type 2 diabetes and is associated with increased E-selectin levels
TLDR
Elevated plasma irisin in T2DM is associated with indices of adiposity, and that irisin may be involved in pro-atherogenic endothelial disturbances that accompany obesity and T2 DM is suggested. Expand
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References

SHOWING 1-10 OF 33 REFERENCES
Irisin – a myth rather than an exercise-inducible myokine
TLDR
The results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species. Expand
Evidence against a Beneficial Effect of Irisin in Humans
TLDR
It is rather unlikely that the beneficial effect of irisin observed in mice can be translated to humans, according to analyses of genomic DNA, mRNA and expressed sequence tags. Expand
Resistance exercise training does not affect plasma irisin levels of hemodialysis patients.
  • C. Moraes, V. Leal, +4 authors D. Mafra
  • Medicine
  • Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme
  • 2013
TLDR
Assessment of the effect of an intradialytic resistance exercise training program (RETP) on plasma irisin levels of hemodialysis (HD) patients and healthy subjects found that HD patients seem to have lower Plasma irisin when compared to healthy subjects. Expand
Exercise-induced irisin secretion is independent of age or fitness level and increased irisin may directly modulate muscle metabolism through AMPK activation.
TLDR
In vitro experiments showed that irisin facilitates glucose and lipid metabolism in human muscle through AMP kinase phosphorylation, and exercise-induced irisin secretion is independent of age or fitness level. Expand
Relationship between serum irisin levels and urinary albumin excretion in patients with type 2 diabetes.
TLDR
Cutting plasma levels of irisin seem to be associated with UAE and FMD in patients with type 2 diabetes, and Pearson's correlation analyses showed a negative correlation between irisin and logUAE and between FMD and log UAE, and positive correlations betweenirisin and F MD. Expand
Effects of obesity, diabetes and exercise on Fndc5 gene expression and irisin release in human skeletal muscle and adipose tissue: in vivo and in vitro studies
Considerable controversy exists regarding the role of irisin, a putative exercise‐induced myokine, in human metabolism. We therefore studied irisin and its precursor Fndc5 in obesity, type 2 diabetesExpand
Irisin as a muscle-derived hormone stimulating thermogenesis – A critical update
TLDR
The present review will describe the discovery of irisin, its potential role in adipose tissue-mediated thermogenesis, its regulation by exercise and lastly, discuss current controversies and highlight gaps of knowledge to be filled by future studies. Expand
Plasma irisin levels progressively increase in response to increasing exercise workloads in young, healthy, active subjects.
TLDR
Circulating irisin levels were acutely elevated in response to exercise, with a greater increase after maximal workload, suggesting that irisin release could be a function of muscle energy demand. Expand
A PGC1α-dependent myokine that drives browning of white fat and thermogenesis
TLDR
It is shown in mouse that PGC1-α expression in muscle stimulates an increase in expression of FNDC5, a membrane protein that is cleaved and secreted as a newly identified hormone, irisin, which results in improvements in obesity and glucose homeostasis. Expand
Irisin and FGF21 are cold-induced endocrine activators of brown fat function in humans.
TLDR
Results suggest exercise-induced irisin secretion could have evolved from shivering-related muscle contraction, serving to augment brown fat thermogenesis in concert with FGF21, a brown adipokine in rodents. Expand
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