Detection and Characterization of Fungal Infections of Ammophila arenaria ( Marram Grass ) Roots by Denaturing Gradient Gel Electrophoresis of Specifically Amplified 18 S rDNA

@inproceedings{Kowalchuk1997DetectionAC,
  title={Detection and Characterization of Fungal Infections of Ammophila arenaria ( Marram Grass ) Roots by Denaturing Gradient Gel Electrophoresis of Specifically Amplified 18 S rDNA},
  author={George A Kowalchuk and Saskia Gerards and J W Woldendorp},
  year={1997}
}
Marram grass (Ammophila arenaria L.), a sand-stabilizing plant species in coastal dune areas, is affected by a specific pathosystem thought to include both plant-pathogenic fungi and nematodes. To study the fungal component of this pathosystem, we developed a method for the cultivation-independent detection and characterization of fungi infecting plant roots based on denaturing gradient gel electrophoresis (DGGE) of specifically amplified DNA fragments coding for 18S rRNA (rDNA). A nested PCR… CONTINUE READING

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