Detection, sizing, and quantitation of polyadenylated ribonucleic acid in the nanogram-picogram range.

Abstract

A method is described for using very high specific activity [3H]poly(deoxythymidylate) [[3H]poly(dT)] to detect, size, and quantiate subnanogram amounts of nonradioactive polyadenylated RNA. Short (approximately 100 nucleotides long) [3H]poly(dT) is hybridized to the poly(adenylate) [poly(A)] tracts in polyadenylated RNAs. The RNA may then be sized and… (More)

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Cite this paper

@article{Tracy1980DetectionSA, title={Detection, sizing, and quantitation of polyadenylated ribonucleic acid in the nanogram-picogram range.}, author={Sally Tracy and David Kohne}, journal={Biochemistry}, year={1980}, volume={19 16}, pages={3792-9} }