Detection, expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR

@article{Panicker2009DetectionEA,
  title={Detection, expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR},
  author={Gitika Panicker and Nazia Mojib and Jackie M. Aislabie and Asim K Bej},
  journal={Antonie van Leeuwenhoek},
  year={2009},
  volume={97},
  pages={275-287}
}
In this study, 28 hydrocarbon-degrading bacterial isolates from oil-contaminated Antarctic soils were screened for the presence of biodegradative genes such as alkane hydroxylase (alks), the ISPα subunit of naphthalene dioxygenase (ndoB), catechol 2,3-dioxygenase (C23DO) and toluene/biphenyl dioxygenase (todC1/bphA1) by using polymerase chain reaction (PCR) methods. All naphthalene degrading bacterial isolates exhibited the presence of a 648 bp amplicon that shared 97% identity to a known ndoB… CONTINUE READING
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