Detecting transient intermediates in macromolecular binding by paramagnetic NMR

@article{Iwahara2006DetectingTI,
  title={Detecting transient intermediates in macromolecular binding by paramagnetic NMR},
  author={Junji Iwahara and G. Marius Clore},
  journal={Nature},
  year={2006},
  volume={440},
  pages={1227-1230}
}
Macromolecular complex formation is governed by two opposing constraints of specificity and speed. Kinetic and theoretical considerations suggest that significant rate enhancement can be achieved either by reducing the dimensionality of the search process or by the creation of a short-range attractive potential around the target site. This implies the existence of transient intermediates involving non-specific binding modes. Here we show that intermolecular paramagnetic relaxation enhancement… Expand
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