Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR.

@article{Medici2001DetectingTP,
  title={Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR.},
  author={Dario de Medici and Luciana Croci and Simona Di Pasquale and Alfonsina Fiore and Laura Toti},
  journal={Letters in applied microbiology},
  year={2001},
  volume={33 5},
  pages={362-6}
}
AIMS The objective of this study was to determine the presence of infectious hepatitis A virus (HAV) in molluscs naturally contaminated with viral HAV-RNA. METHODS AND RESULTS One hundred and forty-two mollusc samples were analysed for the presence of viral HAV-RNA using RT-nested-PCR; positive samples were then analysed with an integrated method, cell-culture RT-PCR, to detect infectious virus. Viral HAV-RNA was detected in 34.5% of the samples while 12.7% of the total samples were positive… CONTINUE READING

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