Detecting synaptic connections in the medial nucleus of the trapezoid body using calcium imaging

@article{Billups2002DetectingSC,
  title={Detecting synaptic connections in the medial nucleus of the trapezoid body using calcium imaging},
  author={Brian Billups and Amy Wong and I. M. Forsythe},
  journal={Pfl{\"u}gers Archiv},
  year={2002},
  volume={444},
  pages={663-669}
}
The study of synaptic transmission in brain slices generally entails the patch-clamping of postsynaptic neurones and stimulation of identified presynaptic axons using a remote electrical stimulating electrode. Although patch recording from postsynaptic neurones is routine, many presynaptic axons take tortuous turns and are severed in the slicing procedure, blocking propagation of the action potential to the synaptic terminal and preventing synaptic stimulation. Here we demonstrate a method of… CONTINUE READING
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