Detecting single base substitutions as heteroduplex polymorphisms.

  title={Detecting single base substitutions as heteroduplex polymorphisms.},
  author={Marga B. White and M. Carvalho and David Derse and Stephen J. O'Brien and M. Dean},
  volume={12 2},
We have developed a sensitive technique for detecting single base substitutions in polymerase chain reaction (PCR) products from individuals heterozygous for polymorphisms or new mutations. This technique takes advantage of the formation of heteroduplexes in the PCR between different alleles from heterozygous individuals. These heteroduplexes can be detected on polyacrylamide gels because they migrate slower than their corresponding homoduplexes. Using PCR, we have generated a series of point… CONTINUE READING
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