Detecting protein–protein interactions by far western blotting

@article{Wu2007DetectingPI,
  title={Detecting protein–protein interactions by far western blotting},
  author={Yuliang Wu and Qiang Li and X. Chen},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={3278-3284}
}
Far western blotting (WB) was derived from the standard WB method to detect protein–protein interactions in vitro. In Far WB, proteins in a cell lysate containing prey proteins are firstly separated by SDS or native PAGE, and transferred to a membrane, as in a standard WB. The proteins in the membrane are then denatured and renatured. The membrane is then blocked and probed, usually with purified bait protein(s). The bait proteins are detected on spots in the membrane where a prey protein is… CONTINUE READING
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Protein interaction technologies

  • E. A. Golemis, P. D. Adams
  • Protein – Protein Interactions : A Molecular…
  • 2005

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