Detecting ordered domain formation (lipid rafts) in model membranes using Tempo.

@article{Bakht2007DetectingOD,
  title={Detecting ordered domain formation (lipid rafts) in model membranes using Tempo.},
  author={Omar Bakht and Erwin London},
  journal={Methods in molecular biology},
  year={2007},
  volume={398},
  pages={29-40}
}
Short-range fluorescence quenching has proven to be an effective method to detect the presence of coexisting ordered and disordered state lipid domains in model membranes. In this approach a fluorescent group and fluorescence-quenching molecule are incorporated into the lipid bilayer of interest. In a typical experiment, the fluorophore chosen partitions into ordered domains to a significant degree, whereas the quencher partitions more favorably into disordered domains. Thus, in the presence of… CONTINUE READING