Detecting avian malaria: an improved polymerase chain reaction diagnostic.

  title={Detecting avian malaria: an improved polymerase chain reaction diagnostic.},
  author={Sylvia M Fallon and Robert E. Ricklefs and Bill Swanson and Eldredge P Bermingham},
  journal={The Journal of parasitology},
  volume={89 5},
We describe a polymerase chain reaction (PCR) assay that detects avian malarial infection across divergent host species and parasite lineages representing both Plasmodium spp. and Haemoproteus spp. The assay is based on nucleotide primers designed to amplify a 286-bp fragment of ribosomal RNA (rRNA) coding sequence within the 6-kb mitochondrial DNA malaria genome. The rRNA malarial assay outperformed other published PCR diagnostic methods for detecting avian infections. Our data demonstrate… CONTINUE READING