Detecting and resolving position-dependent temperature effects in real-time quantitative polymerase chain reaction.


Real-time quantitative polymerase chain reaction (qPCR) depends on precise temperature control of the sample during cycling. In the current study, we investigated how temperature variation in plate-based qPCR instruments influences qPCR results. Temperature variation was measured by amplicon melting analysis as a convenient means to assess well-to-well… (More)
DOI: 10.1016/j.ab.2011.08.041


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