Detecting and resolving position-dependent temperature effects in real-time quantitative polymerase chain reaction.

Abstract

Real-time quantitative polymerase chain reaction (qPCR) depends on precise temperature control of the sample during cycling. In the current study, we investigated how temperature variation in plate-based qPCR instruments influences qPCR results. Temperature variation was measured by amplicon melting analysis as a convenient means to assess well-to-well… (More)
DOI: 10.1016/j.ab.2011.08.041

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.

Slides referencing similar topics