Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography.

@article{Shaw1997DetectingAC,
  title={Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography.},
  author={Paul D. Shaw and Guichen Ping and S L Daly and Chun Nam Cha and John E. Cronan and Kenneth L. Jun. Rinehart and Stephen K Farrand},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 12},
  pages={
          6036-41
        }
}
Many Gram-negative bacteria regulate gene expression in response to their population size by sensing the level of acyl-homoserine lactone signal molecules which they produce and liberate to the environment. We have developed an assay for these signals that couples separation by thin-layer chromatography with detection using Agrobacterium tumefaciens harboring lacZ fused to a gene that is regulated by autoinduction. With the exception of N-butanoyl-L-homoserine lactone, the reporter detected… CONTINUE READING

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