Detecting alternatively spliced transcript isoforms from single-molecule long-read sequences without a reference genome.

@article{Liu2017DetectingAS,
  title={Detecting alternatively spliced transcript isoforms from single-molecule long-read sequences without a reference genome.},
  author={Xiaoxian Liu and Wenbin Mei and Pamela S. Soltis and Douglas Edward Soltis and William Brad Barbazuk},
  journal={Molecular ecology resources},
  year={2017},
  volume={17 6},
  pages={
          1243-1256
        }
}
Alternative splicing (AS) is a major source of transcript and proteome diversity, but examining AS in species without well-annotated reference genomes remains difficult. Research on both human and mouse has demonstrated the advantages of using Iso-Seq™ data for isoform-level transcriptome analysis, including the study of AS and gene fusion. We applied Iso-Seq™ to investigate AS in Amborella trichopoda, a phylogenetically pivotal species that is sister to all other living angiosperms. Our data… CONTINUE READING
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