Desulfotomaculum genus- and subgenus-specific 16S rRNA hybridization probes for environmental studies.

@article{Hristova2000DesulfotomaculumGA,
  title={Desulfotomaculum genus- and subgenus-specific 16S rRNA hybridization probes for environmental studies.},
  author={Krassimira R. Hristova and Margit Mau and Dasheng Zheng and Rustam I. Aminov and R. I. Mackie and H. Rex Gaskins and Lutgarde M. Raskin},
  journal={Environmental microbiology},
  year={2000},
  volume={2 2},
  pages={
          143-59
        }
}
Based on comparative analysis of 16S rRNA sequences and the recently established phylogeny of the genus Desulfotomaculum, a set of phylogenetically nested hybridization probes was developed and characterized. A genus-specific probe targets all known Desulfotomaculum species (with the exception of Desulfotomaculum acetoxidans), and five specific probes target subclusters within the Desulfotomaculum genus. The dissociation temperature of each probe was determined experimentally. Probe… CONTINUE READING
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