Destabilization of secondary structure in 16S ribosomal RNA by dimethylation of two adjacent adenosines.

@article{Charldorp1981DestabilizationOS,
  title={Destabilization of secondary structure in 16S ribosomal RNA by dimethylation of two adjacent adenosines.},
  author={R van Charldorp and Hans A Heus and P H. Van Knippenberg and J. J. M. Joordens and S H de Bruin and Cornelis W. Hilbers},
  journal={Nucleic acids research},
  year={1981},
  volume={9 17},
  pages={
          4413-22
        }
}
Fragments comprising the 49 nucleotides from the 3'-end have been purified from 16S ribosomal RNA of wild-type Escherichia coli and from a kasugamycin-resistant mutant that specifically lacks dimethylation of two adjacent adenines near the 3'-terminus. These fragments, obtained after treatment of ribosomes in vitro with the bacteriocin cloacin DF13, were used to study the effect of the methyl groups on the temperature dependent unfolding of double-stranded regions. Both fragments contain at… CONTINUE READING
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