Destabilization of interleukin-6 mRNA requires a putative RNA stem-loop structure, an AU-rich element, and the RNA-binding protein AUF1.

@article{Paschoud2006DestabilizationOI,
  title={Destabilization of interleukin-6 mRNA requires a putative RNA stem-loop structure, an AU-rich element, and the RNA-binding protein AUF1.},
  author={Serge Paschoud and Afzal Muhammad Dogar and Catherine Kuntz and Barbara Grisoni-Neupert and Larry H. Richman and Lukas C. K{\"u}hn},
  journal={Molecular and cellular biology},
  year={2006},
  volume={26 22},
  pages={8228-41}
}
Interleukin-6 mRNA is unstable and degraded with a half-life of 30 min. Instability determinants can entirely be attributed to the 3' untranslated region. By grafting segments of this region to stable green fluorescent protein mRNA and subsequent scanning mutagenesis, we have identified two conserved elements, which together account for most of the instability. The first corresponds to a short noncanonical AU-rich element. The other, 80 nucleotides further 5', comprises a sequence predicted to… CONTINUE READING

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