Corpus ID: 20078385

Destabilase-lysozyme-2 - original recombinant thrombolytic preparation of medicinal leech inhibits horse platelets aggregation.

@article{Rotkina2016Destabilaselysozyme2O,
  title={Destabilase-lysozyme-2 - original recombinant thrombolytic preparation of medicinal leech inhibits horse platelets aggregation.},
  author={A. S. Rotkina and Irina V Pronina and Vassili N. Lazarev and D. N. Akhaev and Isolda P. Baskova},
  journal={Patologicheskaia fiziologiia i eksperimental'naia terapiia},
  year={2016},
  volume={60 3},
  pages={
          47-51
        }
}
The purpose. Identifying the capacity of the medicinal leech novel original recombinant thrombolytic preparation Destabilase-Lysozyme-2 to inhibit the blood platelet aggregation. Methods Gene of destabilase-lysozyme. ds2 (mlDL-Ds2 ), was cloned in E.coli cells. Recombinant protein was isolated in denaturing conditions using metal-chelate chromatography followed by denaturation of the polypeptide by rapid dilution in exact accordance with the procedure described by Kurdyumov A.S. et al. ( 2016… Expand
Role of isopeptidolysis in the process of thrombolysis.
TLDR
Cumulatively, it is found that the medicinal leech destabilase is a more efficient thrombolytic agent for dissolving thrombi, which could help increase the overall effectiveness of conventional throm bolytic drugs. Expand