Desorption of immunoglobulins from Protein A-Sepharose CL-4B under mild conditions.


Conditions which permit the dissociation of IgG-staphylococcal protein A interactions without resort to low pH buffers or the use of chaotropic ions are discussed in relation to: (a) the mechanism of the binding reaction; (b) the use of immobilised protein A for purification of cells or removal of immune complexes from serum. Glycyl-tyrosine, glycyl… (More)