Desorption of immunoglobulins from Protein A-Sepharose CL-4B under mild conditions.

@article{Bywater1983DesorptionOI,
  title={Desorption of immunoglobulins from Protein A-Sepharose CL-4B under mild conditions.},
  author={R. E. H. Bywater and G B Eriksson and Tony Ottosson},
  journal={Journal of immunological methods},
  year={1983},
  volume={64 1-2},
  pages={1-6}
}
Conditions which permit the dissociation of IgG-staphylococcal protein A interactions without resort to low pH buffers or the use of chaotropic ions are discussed in relation to: (a) the mechanism of the binding reaction; (b) the use of immobilised protein A for purification of cells or removal of immune complexes from serum. Glycyl-tyrosine, glycyl… CONTINUE READING