Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins.

@article{Fujiwara2015Desmocollin2AF,
  title={Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins.},
  author={Miwako Fujiwara and Azusa Nagatomo and Megumi Tsuda and Shuichi Obata and Tetsushi Sakuma and Takashi Yamamoto and Shintaro Suzuki},
  journal={Journal of biochemistry},
  year={2015},
  volume={158 4},
  pages={339-53}
}
The role of the juxtamembrane region of the desmocollin-2 cytoplasmic domain in desmosome formation was investigated by using gene knockout and reconstitution experiments. When a deletion construct of the desmocollin-2 juxtamembrane region was expressed in HaCaT cells, the mutant protein became localized linearly at the cell-cell boundary, suggesting the involvement of this region in desmosomal plaque formation. Then, desmocollin-2 and desmoglein-2 genes of epithelial DLD-1 cells were ablated… CONTINUE READING