Desmocalmin: a calmodulin-binding high molecular weight protein isolated from desmosomes

@article{Tsukita1985DesmocalminAC,
  title={Desmocalmin: a calmodulin-binding high molecular weight protein isolated from desmosomes},
  author={Shoichiro Tsukita},
  journal={The Journal of Cell Biology},
  year={1985},
  volume={101},
  pages={2070 - 2080}
}
A unique high molecular weight protein (240,000 mol wt) has been purified from isolated desmosomes of bovine muzzle epidermis, using low-salt extraction at pH 9.5-10.5 and gel-filtration followed by calmodulin-affinity column chromatography. This protein was shown to bind to calmodulin in a Ca2+-dependent manner, so we called it desmocalmin here. Desmocalmin also bound to the reconstituted keratin filaments in vitro in the presence of Mg2+, but not to actin filaments. By use of the antibody… CONTINUE READING