Designing conditions for in vitro formation of amyloid protofilaments and fibrils.

Abstract

We have been able to convert a small alpha/beta protein, acylphosphatase, from its soluble and native form into insoluble amyloid fibrils of the type observed in a range of pathological conditions. This was achieved by allowing slow growth in a solution containing moderate concentrations of trifluoroethanol. When analyzed with electron microscopy, the… (More)

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Cite this paper

@article{Chiti1999DesigningCF, title={Designing conditions for in vitro formation of amyloid protofilaments and fibrils.}, author={Fabio Chiti and Paul Webster and Niccolo' Taddei and A. Clark and Micaela Stefani and G. Ramponi and C. Melissa Dobson}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1999}, volume={96 7}, pages={3590-4} }