# Designing Heterodimeric Two-stranded α-Helical Coiled-coils

@article{Litowski2002DesigningHT,
title={Designing Heterodimeric Two-stranded $\alpha$-Helical Coiled-coils},
author={J. Litowski and R. Hodges},
journal={The Journal of Biological Chemistry},
year={2002},
volume={277},
pages={37272 - 37279}
}
• Published 2002
• Chemistry
• The Journal of Biological Chemistry
The E/K coil, a heterodimeric coiled-coil, has been designed as a universal peptide capture and delivery system for use in applications such as biosensors and as an expression and affinity purification tag. In this design, heterodimer formation is specified through the placement of charged residues at the e and g positions of the heptad repeat such that the E coil contains all glutamic acid residues at these positions, and the K coil contains all lysine residues at these positions. The affinity… Expand
195 Citations

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