Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells

Abstract

The pyrrolysyl-tRNA synthetase/tRNAPyl pair is the most versatile and widespread system for the incorporation of non-canonical amino acids (ncAAs) into proteins in mammalian cells. However, low yields of ncAA incorporation severely limit its applicability to relevant biological targets. Here, we generate two tRNAPyl variants that significantly boost the… (More)
DOI: 10.1093/nar/gkx1156

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