Designer proteins: applications of genetic code expansion in cell biology

@article{Davis2012DesignerPA,
  title={Designer proteins: applications of genetic code expansion in cell biology},
  author={Lloyd Davis and Jason W Chin},
  journal={Nature Reviews Molecular Cell Biology},
  year={2012},
  volume={13},
  pages={168-182}
}
Designer amino acids, beyond the canonical 20 that are normally used by cells, can now be site-specifically encoded into proteins in cells and organisms. This is achieved using 'orthogonal' aminoacyl-tRNA synthetase–tRNA pairs that direct amino acid incorporation in response to an amber stop codon (UAG) placed in a gene of interest. Using this approach, it is now possible to study biology in vitro and in vivo with an increased level of molecular precision. This has allowed new biological… CONTINUE READING

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