Designer diatom episomes delivered by bacterial conjugation

  title={Designer diatom episomes delivered by bacterial conjugation},
  author={Bogumil J. Karas and Rachel E. Diner and Stephane C. Lefebvre and Jeff McQuaid and Alex P.R. Phillips and Chari M. Noddings and John K Brunson and Ruben E. Valas and Thomas J. Deerinck and Jelena Jablanovic and Jeroen T. F. Gillard and Karen Beeri and Mark H. Ellisman and John I. Glass and Clyde A. Hutchison and Hamilton O. Smith and J. Craig Venter and Andrew E Allen and Christopher L. Dupont and Philip D Weyman},
  booktitle={Nature communications},
Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value chemicals. However, compared with model genetic organisms such as Escherichia coli and Saccharomyces cerevisiae, characterization of the complex biology and biochemistry of algae and strain improvement has been hampered by the inefficient genetic tools. To date, many algal species are transformable only via particle bombardment, and the introduced DNA is integrated randomly into the nuclear genome. Here we… CONTINUE READING
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